Cloned (Comment) | Organism |
---|---|
gene rv3229c, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli as c-Myc and/or His-tagged protein, DesA3 expressed as a fusion with either a C-terminal His6 or c-myc tag has consistently higher activity and stability than native DesA3 having the native C-terminal sequence of LAA. The native C-terminal sequence of LAA apparently serves as a binding determinant for a mycobacterial protease/degradation system directed at DesA3. Fusion of only the last 12 residues of native DesA3 to the C-terminal of GFP is sufficient to make GFP unstable | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | stability and activity of several GFP-fusion mutant enzymes variants, resistance os sensitivity to mycobacterial proteases, overview | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral | Mycobacterium tuberculosis | 16020 | - |
microsome | - |
Mycobacterium tuberculosis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
x * 75000, about, recombinant untagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycobacterium tuberculosis | DesA3 is a membrane-bound stearoyl-CoA desaturase that works together with oxidoreductase Rv3230c, encoded by gene rv3230c, to produce oleic acid, an 55 essential constituent of mycobacterial membrane phospholipids and triglycerides | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | DesA3 is a membrane-bound stearoyl-CoA desaturase that works together with oxidoreductase Rv3230c, encoded by gene rv3230c, to produce oleic acid, an 55 essential constituent of mycobacterial membrane phospholipids and triglycerides | ? | - |
? | |
stearoyl-CoA + ferrocytochrome b5 + O2 + H+ | Mycobacterium tuberculosis | essential enzyme activity, regulation mechanism, overview | oleoyl-CoA + ferricytochrome b5 + H2O | - |
? | |
stearoyl-CoA + ferrocytochrome b5 + O2 + H+ | Mycobacterium tuberculosis H37Rv | essential enzyme activity, regulation mechanism, overview | oleoyl-CoA + ferricytochrome b5 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene rv3229c | - |
Mycobacterium tuberculosis H37Rv | - |
gene rv3229c | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | DesA3 is a membrane-bound stearoyl-CoA desaturase that works together with oxidoreductase Rv3230c, encoded by gene rv3230c, to produce oleic acid, an 55 essential constituent of mycobacterial membrane phospholipids and triglycerides | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme reacts with oxidoreductase Rv3230c to produce oleic acid | Mycobacterium tuberculosis | ? | - |
? | |
additional information | DesA3 is a membrane-bound stearoyl-CoA desaturase that works together with oxidoreductase Rv3230c, encoded by gene rv3230c, to produce oleic acid, an 55 essential constituent of mycobacterial membrane phospholipids and triglycerides | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | the enzyme reacts with oxidoreductase Rv3230c to produce oleic acid | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
stearoyl-CoA + ferrocytochrome b5 + O2 + H+ | essential enzyme activity, regulation mechanism, overview | Mycobacterium tuberculosis | oleoyl-CoA + ferricytochrome b5 + H2O | - |
? | |
stearoyl-CoA + ferrocytochrome b5 + O2 + H+ | DesA3 expressed as a fusion with either a C-terminal His6 or c-myc tag has consistently higher activity and stability than native DesA3 having the native C-terminal sequence of LAA | Mycobacterium tuberculosis | oleoyl-CoA + ferricytochrome b5 + H2O | - |
? | |
stearoyl-CoA + ferrocytochrome b5 + O2 + H+ | essential enzyme activity, regulation mechanism, overview | Mycobacterium tuberculosis H37Rv | oleoyl-CoA + ferricytochrome b5 + H2O | - |
? | |
stearoyl-CoA + ferrocytochrome b5 + O2 + H+ | DesA3 expressed as a fusion with either a C-terminal His6 or c-myc tag has consistently higher activity and stability than native DesA3 having the native C-terminal sequence of LAA | Mycobacterium tuberculosis H37Rv | oleoyl-CoA + ferricytochrome b5 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, about, recombinant untagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
DesA3 | - |
Mycobacterium tuberculosis |
membrane-bound stearoyl-CoA DELTA9 desaturase | - |
Mycobacterium tuberculosis |
stearoyl-CoA desaturase | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ferrocytochrome b5 | - |
Mycobacterium tuberculosis |